Streptomycin-resistant Escherichia coli mutant temperature sensitive for the production of Qbeta-infective particles
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Streptomycin-resistant Escherichia coli mutant temperature sensitive for the production of Qbeta-infective particles.
A streptomycin-resistant Escherichia coli mutant has been isolated that is temperature sensitive for Qbeta phage, but not for the group I RNA phages f2, MS2, and R17. The growth of Qbeta in the mutant at the nonpermissive temperature (42 degrees C) results in the release of a near-normal burst of noninfectious particles that cosediment with Qbeta in a sucrose gradient. It is assumed that the mu...
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The temperature-sensitive mutation in our Escherichia coli strain C1714 appears to be in a ribosomal protein. The mutation maps at the streptomycin locus. Protein synthesis in intact cells stops immediately when the temperature is shifted from 30 to 42 degrees C. Analysis of polyribosome distributions after shift-up suggests that initiation of protein synthesis is defective at the high temperat...
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A mutant sensitive to colicin M at 30 degrees C and tolerant at 42 degrees C to high concentrations of colicin M was isolated from Escherichia coli K-12. A temperature shift from 30 to 42 degrees C rescued all cells up to the time they started to lyse at 30 degrees C (25 min after addition of colicin M). The growth rate at 42 degrees C remained unaffected by colicin M. AT 42 degrees C the cell-...
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t Davis, B. D., Bact. Proc., 1952, 157. 1 Selbie, F. R., Brit. Med. Bull., 4, 267 (1946-1947). 2 Work, T. S., and Work, E., The Basis of Chemotherapy, Interscience, New York, 1948, Ch. VI. 3Davis, B. D., J. Exp. Med., 94, 243 (1951). 4Davis, B. D., and Mingioli, E. S., J. Bact., 60, 17 (1950). 6 N-(2-thiazolyl)-4-hydroxybenzene sulfonamide. This compound was kindly furnished by the American Cya...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1977
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.21.1.1-6.1977